Stimulation of the hexose monophosphate shunt in human neutrophils by ascorbic acid: mechanism of action.
نویسندگان
چکیده
The addition of either ascorbic acid or dehydroascorbic acid to a suspension of polymorphonuclear leukocytes caused a dramatic increase in the resting hexose monophosphate shunt activity. A sequence of reactions involving dehydroascorbate, reduced glutathione, and reduced nicotinamide adenine dinucleotide phosphate is described to explain this stimulation. This sequence could provide an alternate method of producing H(2)O(2) and a bactericidal mechanism which is independent of myeloperoxidase.
منابع مشابه
Stimulation of leukocyte hexose monophosphate shunt activity by ascorbic Acid.
Ascorbic acid in vitro markedly stimulates the hexose monophosphate shunt in normal human neutrophils, rabbit alveolar macrophages, and in neutrophils from a patient with chronic granulomatous disease.
متن کاملEnhancement of random migration and chemotactic response of human leukocytes by ascorbic acid.
Incubation of human leukocytes with ascorbic acid at neutral pH and at concentrations 10-50 times that of normal blood levels augmented both the in vitro random migration and chemotaxis of the cells by 100-300% without influencing their phagocytic capacity. Enhancement of mobility by ascorbate was evident for isolated neutrophils, eosinophils, and mono-nuclear leukocytes and was independent of ...
متن کاملHuman neutrophil migratory function: modulatory effect of interactions with opsonized particles.
Human polymorphonuclear neutrophils preexposed to cytotaxin or to phagocytizable particles exhibited reduced spontaneous and chemotactic migratory responses. This influence of cytotaxin appears to be related to toxic effects of by-products of hexose monophosphate shunt stimulation. To determine whether a phagocytic stimulus may inhibit subsequent neutrophil migratory functions by the same mecha...
متن کاملThe particulate superoxide-forming system from human neutrophils. Properties of the system and further evidence supporting its participation in the respiratory burst.
Studies were performed to characterize the previously reported particulate O2--forming system from human neutrophils. Of eight reducing agents examined, including glutathione, ascorbic acid, and intermediates of the glycolytic and hexose monophosphate shunt pathways, only the pyridine nucleotides could serve as electron donors. At 0.1 mM pyridine nucleotide, O2- production was relatively indepe...
متن کاملComparison of human eosinophils from normals and patients with eosinophilia.
Previous studies of the biochemistry and physiology of eosinophils have relied upon cells obtained from patients with eosinophilia (EE). It is unknown whether such cells might have been activated or partially exhausted by the pathological state causing eosinophilia. We examined cell surface charge, membrane transport of deoxyglucose, activation of lyso-somal acid phosphatase, and oxidative meta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 1 1 شماره
صفحات -
تاریخ انتشار 1972